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The type II and III neuraminidase preparations from human tear fluid and lung, respectively, show a temperature-dependent two-step reaction when poly(N-acetyl-D-glucosamine) is used as a substrate. The first step is the hydrolysis of the exo-linkage releasing the terminal sialic acid (N-acetylneuraminic acid), and the second step, after pre-adsorption of the released sialic acid on the polymeric substrate, is the hydrolysis of the terminal sialic acid from the polymeric substrate. The two types of enzyme preparations are thus equally active when the polymeric substrate is the polysialic acid isolated from E. coli. This finding may explain why polysialic acid is present in the corneal stroma of the human eye, and in E. coli.Image copyright AFP Image caption Mr Netanyahu is under investigation for alleged corruption
Israeli Prime Minister Benjamin Netanyahu has said he will not step down ahead of national elections next year.
Mr Netanyahu, who faces trial for corruption, accused his potential opponents of seeking to oust him as early as next week's ballot.
"There will be no early elections," he said.
Mr Netanyahu is being investigated for allegedly receiving benefits from an Israeli telecoms firm 0b46394aab